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A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study the properties of organic or inorganic substances. Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation while phosphorescence is a specific type of photoluminescence related to fluorescence. Fluorescence microscope is used to study the absorption and transport of substances within cells, as well as the distribution and positioning of chemical substances. Fluorescence microscopy is the most popular method for studying the dynamic behavior exhibited in live-cell imaging.