Charge Variant Analysis to Characterize mAbs (CAT#: STEM-MB-0139-WXH)

Introduction

The existence of multiple variants with differences in either charge, molecular weight or other properties is a common feature of monoclonal antibodies，which is charge variant.
Charge variant analysis is a widely used analytical tool in characterization of monoclonal antibodies (mAbs).
Biotherapeutic proteins such as monoclonal antibodies (mAbs) are far more heterogeneous than small-molecule drugs. The presence of the charged state can significantly impact the structure, stability, binding affinity, and efficacy of the biotherapeutic drug. It is therefore necessary to understand the profile of the drug so that charge variants are identified and removed if necessary. The heterogeneity of monoclonal antibodies is revealed by charge sensitive methods, such as ion exchange chromatography (IEX).