Cytological diagnosis of pulmonary cancer by fluorescence microscope (CAT#: STEM-MIT-0181-LJX)

Introduction

Lung cancer is a malignant tumor originating from the bronchial mucosa or glands of the lung, with the fastest increasing morbidity and mortality. It is one of the most dangerous malignant tumors to the health and life of the population.

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Principle Applications Procedure Materials Notes

Principle

Fluorescence microscope is a basic tool of immunofluorescence cytochemistry. It is composed of light source, filter plate system and optical system. Is to use a certain wavelength of light to stimulate the specimen to emit fluorescence, through the objective lens and eyepiece system to enlarge the image to observe the specimen.

Applications

Studing the absorption, transportation, distribution and localization of chemical substances in cells
For observation and study of plant cells

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Lung cancer cells

Notes

Operate in strict accordance with the manufacturer's instructions and do not change the procedure at will
The object of observation must be a specimen that fluoresces spontaneously or has been stained with fluorescent dye

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