Examination of bacteria in soil by fluorescence microscope (CAT#: STEM-MIT-0174-LJX)

Introduction

There are many kinds of microorganisms in the soil, including bacteria, fungi, actinomyces, algae and protozoa. Most soil microorganisms are beneficial to crop growth and development, and they have significant effects on soil formation and development, material circulation and fertility evolution.

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Principle Applications Procedure Materials Notes

Principle

Fluorescence microscope is a basic tool of immunofluorescence cytochemistry. It is composed of light source, filter plate system and optical system. Is to use a certain wavelength of light to stimulate the specimen to emit fluorescence, through the objective lens and eyepiece system to enlarge the image to observe the specimen.

Applications

Studing the absorption, transportation, distribution and localization of chemical substances in cells
For observation and study of plant cells

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Bacteria in soil

Notes

Operate in strict accordance with the manufacturer's instructions and do not change the procedure at will
The object of observation must be a specimen that fluoresces spontaneously or has been stained with fluorescent dye

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