Detection of Epidermal Growth Factor Receptor Gene Mutations in Lung Adenocarcinomas by Rapid Polymerase Chain Reaction (CAT#: STEM-MT-0010-LGZ)

Introduction

Somatic mutations in the tyrosine kinase domain of the epidermal growth factor receptor (EGFR) gene are present in lung adenocarcinomas responsive to the EGFR inhibitors gefitinib and erlotinib. Two types of mutations account for 90% of mutation cases: short in-frame deletions of exon 19 and specific point mutations in codon 858 (L858R) of exon 21.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Lung Adenocarcinomas

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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