Detection of the specific Anthrax biomarker-protective antigen (PA) by Surface-enhanced Raman spectroscopy (SERS) (CAT#: STEM-ST-0043-WXH)
Introduction
The protective antigen (PA) component of the anthrax toxins is an essential virulence factor of Bacillus anthracis and is the major protective immunogen.
Surface-enhanced Raman spectroscopy or surface-enhanced Raman scattering (SERS) is a surface-sensitive technique that enhances Raman scattering by molecules adsorbed on rough metal surfaces or by nanostructures such as plasmonic-magnetic silica nanotubes. The enhancement factor can be as much as 1010 to 1011, which means the technique may detect single molecules.
Applications
• Analysis of biocompatibility of a material. • Analysis of nucleic acids. • Study of interactions between drugs and cells. • Photodynamic therapy (PDT). • Analyzing metabolic accumulations of a substance or compounds. • Diagnosis of disease. • Analysis of individual cells. • Cell sorting applications. • Analyzing the features of biomolecules. • Study of bone structure.
Procedure
1. Preparation of samples 2. Determine instrument parameters 3. Perform background scan 4. Test the sample 5. Data analysis