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Examination of change in molecular structure of intact moisten nails by Raman spectroscopy (CAT#: STEM-ST-0035-WXH)

Introduction

It was believed that the physical properties of nails changes because of change in keratin structure.
Keratin is a protein that helps form hair, nails and your skin's outer layer (epidermis). It helps support your skin, heal wounds and keep your nails and hair healthy. There are 54 kinds of keratin in your body.




Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope
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