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Dial-out PCR (CAT#: STEM-MB-0226-WXH)

Introduction

Dial-out PCR is a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequences by PCR. Dial-out PCR enables multiplex in vitro clone screening and is a compelling alternative to in vivo cloning and Sanger sequencing for accurate gene synthesis.




Applications

Rapid and accurate gene synthesis.

Procedure

General procedure:
1. Denaturation
2. Annealing
3. Extension