Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Dynamic light scattering (DLS) to determine the size of particles (CAT#: STEM-MB-0239-WXH)

Introduction

Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS) or quasi-elastic light scattering (QELS), is one of the most common analytical methods used to determine the size and radius distribution of particles in suspension or in polymers.
In dynamic light scattering, the speed at which the particles diffuse due to Brownian motion is measured. This is done by determining the rate at which the intensity of the scattered light fluctuates when detected using a suitable optical arrangement.




Applications

• Study of homogeneity of protein molecules
• Study of pH stability of protein molecules
• Study of thermal stability of protein molecules
• Study of protein denaturation and folding
• Particle size distribution analysis
• Protein–protein, Protein–RNA, Protein–small molecules interaction studies
• Detecting aggregation of recombinant proteins

Procedure

1. Sample preparation
2. Detection using dynamic light scattering
2. Detection by differential scanning calorimeter
3. Data analysis
Advertisement