Epoxy-Activated Agarose (Dry Form), 15 g, Resin Active Group Epoxy, 786-1222, G-BIOSCIENCES (CAT#: STEM-C-3100-LGZ)

Epoxy-activated agarose is a pre-activated resin for affinity chromatography. Epoxy-activated agarose consists of a high density of epoxy groups that form covalent bonds with ligands containing amine, sulfhydryl, or hydroxyl groups.

Epoxy-activated agarose is prepared by immobilizing isooxyethers of 1,4-butanediol diglycidyl ether on a matrix. This preactivated resin provides a hydrophilic 12-atom spacer that enables the conjugation of small ligand molecules such as amino acids, monosaccharides, peptides and carbohydrates.

Epoxy-activated agarose is widely used in affinity chromatography, including the purification of antigens, antibodies, lectins, glycoproteins, etc.

Size: 15 g
Resin Active group: epoxy
Resin Active group density: 20-40 µM/ ml hydrated resin.
Group to be coupled: -NH2, -OH or -SH
45-165µm particle size range
Spherical, highly cross-linked 6% agarose
Resin spacer arm: 12 atom hydrophilic spacer arm
Coupling conditions: pH 7.5-8.5 for thiol ligands, pH 9-10 for amine ligands and pH 11-13 for hydroxyl ligands at 4 to 37°C depending upon ligand stability for 16 hrs
pH stability: 2-13, ligand dependent
1 gm of dry resin swells to 5-6 ml.
Storage: 2- 8°C, desiccated

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