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G-Trap™ Protein G, 1 ml, 5 Columns, 786-1033, G-BIOSCIENCES (CAT#: STEM-C-3262-LGZ)

Highlights

G-Trap™ Protein G resin

Mean particle size: 90 µM

Cat Number: STEM-C-3262-LGZ

Application: G-Trap™ Protein G are prepacked ready to use columns for affinity purification of Immunoglobulins G.

Model: 786-1033

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Description

G-trap™ Protein G is pre-packaged and ready-to-use column format for affinity purification of IgG. The columnar structure is filled with a resin that is covalently coupled to recombinant protein G.

Recombinant protein G is covalently coupled to highly cross-linked 6% agarose via a proprietary conjugation method. The employed coupling chemistry has a high coupling efficiency and a low protein G leaching rate, and thus a high binding efficiency. Recombinant protein G produced in E. coli has been engineered to genetically remove the albumin and cell surface binding regions of the protein.

G-Trap™ rProtein A FF, 1 ml and G-Trap™ rProtein A FF, 5 ml can also be used for affinity purification of immunoglobulins.

Specification

Size: 5 columns

Features

G-Trap™ Protein G resin

Mean particle size: 90 µM
Bead Structure: Highly cross-linked 6% agarose
Ligand: Recombinant Streptococcal Protein G lacking the albumin binding domain produced in E. coli
Binding capacity: 38mg human IgG/ml resin ; >20mg sheep IgG/ml resin
Maximum flow rate: 1000 cm/hr
Chemical Stability:Stable in all commonly used buffers
pH stability: Long term: pH 3-9, short term pH 2-9
Storage: 2°C to 8°C in 20% ethanol


G-Trap™ Protein G, 1 ml columns

Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene


G-Trap™ Protein G, 5 ml columns

Column Volume: 5 ml
Columns Dimension: 1.6 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene

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