G-trap™ Protein G is pre-packaged and ready-to-use column format for affinity purification of IgG. The columnar structure is filled with a resin that is covalently coupled to recombinant protein G.
Recombinant protein G is covalently coupled to highly cross-linked 6% agarose via a proprietary conjugation method. The employed coupling chemistry has a high coupling efficiency and a low protein G leaching rate, and thus a high binding efficiency. Recombinant protein G produced in E. coli has been engineered to genetically remove the albumin and cell surface binding regions of the protein.
G-Trap™ rProtein A FF, 1 ml and G-Trap™ rProtein A FF, 5 ml can also be used for affinity purification of immunoglobulins.
Specification
Size: 5 columns
Features
G-Trap™ Protein G resin
Mean particle size: 90 µM Bead Structure: Highly cross-linked 6% agarose Ligand: Recombinant Streptococcal Protein G lacking the albumin binding domain produced in E. coli Binding capacity: 38mg human IgG/ml resin ; >20mg sheep IgG/ml resin Maximum flow rate: 1000 cm/hr Chemical Stability:Stable in all commonly used buffers pH stability: Long term: pH 3-9, short term pH 2-9 Storage: 2°C to 8°C in 20% ethanol
G-Trap™ Protein G, 1 ml columns
Column Volume: 1 ml Columns Dimension: 0.7 x 2.5 cm Column Harware Pressure Limit: 0.5 MPa Column Hardware: Polypropylene
G-Trap™ Protein G, 5 ml columns
Column Volume: 5 ml Columns Dimension: 1.6 x 2.5 cm Column Harware Pressure Limit: 0.5 MPa Column Hardware: Polypropylene
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