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Glutathione Reductase Analysis in Plant Tissues

All kinds of abiotic and biotic stresses significantly limit plant productivity by disturbing cellular biochemistry and physiology via the overproduction of activated oxygen molecule-reactive oxygen species (ROS). Uncontrolled accumulation of ROS results in oxidation of DNA and RNA, protein denaturation and decreased enzyme activity as well as lipid peroxidation and carbohydrate oxidation. Within the antioxidant system in plants, the ascorbate-glutathione (AsA-GSH) path-way plays an important role in regulating and controlling the content of ROS.

Glutathione reductase (GR) belongs to the family of NADPH-dependent oxidoreductases and is located in the chloroplasts, cytosol, mitochondria and peroxisomes. As a GSH-regenerating enzyme of the AsA-GSH cycle, GR plays a critical role in maintaining the reduced glutathione (GSH) pool by catalyzing the conversion of oxidized glutathione (GSSG) to GSH with NADPH as cofactor and electron donor. GR is closely related to a lot of abiotic stress conditions where oxidative stress occurs, such as salinity, drought, UV radiation, high light intensity, chilling and contamination by ozone, heavy metals and herbicides, among others.

STEMart provides comprehensive analysis services to identify and investigate the function of GR in regulating plant environmental stress responses.

Test Samples

Plant tissue

Test Capabilities

  • Identification of GR genes
  • Phylogenetic analysis of GR genes
  • Structure analysis of GR genes
  • Expression profile of GR genes of plant under stress conditions
  • 3D structure and physicochemical properties of GR genes
  • Subcellular localization of GR enzymes and Isoenzymes analysis
  • Activity determination of GR

Why Choose Us

  • Comprehensive test report including relevant experimental steps, relevant parameters of the experiment, original pictures, original data, and complete experiment results
  • Customized service for plant research
  • Professional instruments and equipment for reliable data and results
  • Rapid turnaround and cost-effective

For more information about our plant GR analysis service, please contact us.

References

  1. Apel, K., Hirt, H. (2004). “Reactive oxygen species: metabolism, oxidative stress, and signal transduction”. Annu. Rev. Plant Biol. 55, 373–399.
  2. Foyer C.H., Noctor G. (2011). “Ascorbate and glutathione: the heart of the redox hub”. Plant Physiol. 155(1): 2–18.

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