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His-Tagged Protein Purification Medium (HP-IDA), Polyacrylate Microspheres, Bogen (CAT#: STEM-C-3012-LGZ)

Highlights

His-tagged protein purification column (HP-IDA) uses super-porous polyacrylate microspheres as the matrix and IDA as the metal chelating ligand to chelate Ni2+ (commonly known as nickel filler), and can also chelate Cu2+ according to customer needs , Co2+, Zn2+ plasma groups.

Cat Number: STEM-C-3012-LGZ

Application: The process of separating and purifying histidine (His)-tagged proteins using His-tagged protein purification media usually includes steps such as equilibration, sample loading, washing, elution, and regeneration.

Model: His-Tagged Protein Purification Medium (HP-IDA)

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Description

The His-tagged protein purification medium is suitable for the purification of His-tagged fusion proteins. This product can quickly extract the His-tagged fusion protein from cell fermentation broth.

Specification

Matrix: Polyacrylate Microspheres
Ligand: IDA
Shape: spherical
Particle size range: 70±10 μm
Dynamic capacity: ≥30 mg His-Pro/ml wet gel
Maximum flow rate (25°C): 1000 cm/h
Maximum withstand pressure: 1 MPa
pH stability: 3~13 (long time); 2~14 (short time)
Storage: 4~8 °C (20% ethanol)

Features

His-tagged protein purification column (HP-IDA) uses super-porous polyacrylate microspheres as the matrix and IDA as the metal chelating ligand to chelate Ni2+ (commonly known as nickel filler), and can also chelate Cu2+ according to customer needs , Co2+, Zn2+ plasma groups.

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