His-Tagged Protein Purification Medium (HP-IDA), Polyacrylate Microspheres, Bogen (CAT#: STEM-C-3012-LGZ)

Highlights

His-tagged protein purification column (HP-IDA) uses super-porous polyacrylate microspheres as the matrix and IDA as the metal chelating ligand to chelate Ni2+ (commonly known as nickel filler), and can also chelate Cu2+ according to customer needs , Co2+, Zn2+ plasma groups.

Cat Number: STEM-C-3012-LGZ

Application: The process of separating and purifying histidine (His)-tagged proteins using His-tagged protein purification media usually includes steps such as equilibration, sample loading, washing, elution, and regeneration.

Model: His-Tagged Protein Purification Medium (HP-IDA)

Add to Cart

Description Specification Features Related Products

Description

The His-tagged protein purification medium is suitable for the purification of His-tagged fusion proteins. This product can quickly extract the His-tagged fusion protein from cell fermentation broth.

Specification

Matrix: Polyacrylate Microspheres
Ligand: IDA
Shape: spherical
Particle size range: 70±10 μm
Dynamic capacity: ≥30 mg His-Pro/ml wet gel
Maximum flow rate (25°C): 1000 cm/h
Maximum withstand pressure: 1 MPa
pH stability: 3~13 (long time); 2~14 (short time)
Storage: 4~8 °C (20% ethanol)

Features

Related Products

Antibody Purification Media (ProA), Agarose, Bogen (CAT#-STEM-C-3001-LGZ)
Antibody Purification Media (ProG), Agarose, Bogen (CAT#-STEM-C-3002-LGZ)
Antibody Purification Media (GP-ProA), Polyacrylate, Bogen (CAT#-STEM-C-3003-LGZ)
His-Tagged Protein Purification Media (Plus), Agarose Gel Microspheres, Bogen (CAT#-STEM-C-3004-LGZ)
His-Tagged Protein Purification Medium (NTA), Agarose Gel Microspheres, Bogen (CAT#-STEM-C-3005-LGZ)
His-Tagged Protein Purification Medium (IDA), Agarose Gel Microspheres, Bogen (CAT#-STEM-C-3006-LGZ)

Other recommended products

MagINDEX™ rProtein A Magnetic Agarose, Settled Resin, 2 mL, 11-0271-002, Marvelgent biosciences (CAT#: STEM-C-3699-LGZ)
G-Sep™ DEAE Agarose Fast Flow, 25ml, 786-967, G-BIOSCIENCES (CAT#: STEM-C-3301-LGZ)
Capto MMC, 1 L, Cytiva (CAT#: STEM-C-1562-LC)
G-Trap™ Protein G, 1 ml, 1 Column, 786-1034, G-BIOSCIENCES (CAT#: STEM-C-3260-LGZ)
PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF, Prepacked Cartridge, 5 x 5mL, 11-0230-5x5ML, Marvelgent biosciences (CAT#: STEM-C-3662-LGZ)
Sulfhydryl Immobilization Kit For Proteins, 5 columns, 2-40mg Protein/ Column, 786-804, G-BIOSCIENCES (CAT#: STEM-C-3108-LGZ)
PROTEINDEX™ rProtein G Agarose 4 Fast Flow, 100 mL, 11-0206-100, Marvelgent biosciences (CAT#: STEM-C-3710-LGZ)
Sephadex G-50 Medium, 500 g, Cytiva (CAT#: STEM-C-1608-LC)