Identification of Adeno-Associated Virus Serotypes by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0774-WXH)

Introduction

Recombinant adeno-associated virus (AAV) vectors have evolved as the most promising technology for gene therapy due to their good safety profile, high transduction efficacy, and long-term gene expression in non-dividing cells. AAV-based gene therapy holds great promise for treating genetic disorders like inherited blindness, muscular atrophy, or bleeding disorders. Multiple naturally occurring and engineered AAV serotypes exist, which differ in capsid sequence and as a consequence in cellular tropism. Individual AAV capsids differ in thermal stability and have a characteristic melting temperature (Tm), which enables serotype-specific discrimination of AAV vectors. Different AAV serotypes not only have distinct cellular tropism and transduction profiles, but also differ in their structural properties and stability.