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Laser scanning confocal microscope is a set of observation, analysis and output system that uses laser as light source, adopts conjugation focusing principle and device on the basis of traditional optical microscope, and uses computer to process digital image of the observed object. Through the laser scanning confocal microscope, the observation sample can be tomographically scanned and imaged. Therefore, the three-dimensional spatial structure of cells can be observed and analyzed without damage. At the same time, laser scanning confocal microscopy is also a powerful tool for dynamic observation of living cells, multiple immunofluorescence labeling and ion fluorescent labeling observation.
Compared with the traditional optical microscope, it mainly solves the problem that the structure of biological samples overlaps and affects the observation, and can form a clear three-dimensional image. The main advantages in detection are:
1. Obtain three-dimensional images of fine tissue structures at various levels
2. It can detect the ratio and dynamic change of ion concentration in the cell.
3. Simultaneous multi-substance marking and simultaneous observation can be performed on the same sample;
4. Fast fluorescence detection, precise control of excitation light intensity, small photobleaching and fluorescence quenching;
5. No damage to cell detection, precise, accurate, reliable and excellent repeatability