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Laser Scanning Confocal Microscope for dynamic observation of living cells (CAT#: STEM-PET-0015-WXH)

Introduction

Laser scanning confocal microscope is a set of observation, analysis and output system that uses laser as light source, adopts conjugation focusing principle and device on the basis of traditional optical microscope, and uses computer to process digital image of the observed object. Through the laser scanning confocal microscope, the observation sample can be tomographically scanned and imaged. Therefore, the three-dimensional spatial structure of cells can be observed and analyzed without damage. At the same time, laser scanning confocal microscopy is also a powerful tool for dynamic observation of living cells, multiple immunofluorescence labeling and ion fluorescent labeling observation.
Compared with the traditional optical microscope, it mainly solves the problem that the structure of biological samples overlaps and affects the observation, and can form a clear three-dimensional image. The main advantages in detection are:
1. Obtain three-dimensional images of fine tissue structures at various levels
2. It can detect the ratio and dynamic change of ion concentration in the cell.
3. Simultaneous multi-substance marking and simultaneous observation can be performed on the same sample;
4. Fast fluorescence detection, precise control of excitation light intensity, small photobleaching and fluorescence quenching;
5. No damage to cell detection, precise, accurate, reliable and excellent repeatability




Applications

• Cell biology: cell structure, cytoskeleton, cell membrane structure, fluidity, receptors, organelle structure and distribution changes, apoptosis.
• Biochemistry: enzyme, nucleic acid, FISH (fluorescence in situ hybridization), receptor analysis.
• Pharmacology: the effect of drugs on cells and their kinetics.
• Physiology: membrane receptors, ion channels, intracellular ion content, distribution, dynamics.
• Genetics and histoembryology: cell growth, differentiation, mature changes, three-dimensional structure of cells, chromosome analysis, gene expression, gene diagnosis.
• Neurobiology: structure of nerve cells, components of neurotransmitters, transport and transmission, receptors for transmitters, influx of ions, structure of nerve tissues (such as cerebral cortex), cell distribution.
• Microbiology and parasitology: morphological structure (surface and internal structure) of bacteria and parasites.
• Pathology and clinical application of pathology: rapid diagnosis of biopsy specimens, tumor diagnosis, autoimmune disease diagnosis, HV, cervical epithelial cell smear diagnosis.

Procedure

1. Select the appropriate laser, laser power, spectral filter and emission filter according to the excitation and emission wavelengths of the fluorescent probe.
2. Determine the scanning method: point, line, surface, 3D scanning.
3. Set scan parameters.
4. Get the image and save it.
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