Measurement of Phosphate by Ion Selective Electrode (CAT#: STEM-ACT-0110-WXH)

Introduction

Phosphate is the natural source of phosphorous, an element that provides a quarter of all the nutrients that plants need for their growth and development. Phosphorous is used in many products, and is an essential ingredient in all fertilizers.

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Principle Applications Procedure Materials

Principle

An ideal ion-selective electrode (I.S.E.) consists of a thin membrane across which only the intended ion can be transported. The transport of ions from a high conc. to a low one through a selective binding with some sites within the membrane creates a potential difference.

Applications

• Used for the potentiometric determination of several anions (fluoride, chloride, bromide, iodide ion) and cations (sodium, potassium, calcium ion).
• Used for endpoint detection in potentiometric titration.
• In biochemistry, ion selective electrodes are used for the determination of enzymes and substances which interact with enzymes.

Procedure

1. Preparation of Standards
2. Instrument Setup and Operation
3. Analysis of sample

Materials

Ion Selective Electrode

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