Measurement of TAN (total ammonia nitrogen) by Ion Selective Electrode (CAT#: STEM-ACT-0116-WXH)
Introduction
TAN (total ammonia nitrogen) is the total amount of nitrogen in the forms of NH3 and NH4+ in water. In aquaculture, TAN concentration must be less than 0.5 mg/L.
An ideal ion-selective electrode (I.S.E.) consists of a thin membrane across which only the intended ion can be transported. The transport of ions from a high conc. to a low one through a selective binding with some sites within the membrane creates a potential difference.
Applications
• Used for the potentiometric determination of several anions (fluoride, chloride, bromide, iodide ion) and cations (sodium, potassium, calcium ion). • Used for endpoint detection in potentiometric titration. • In biochemistry, ion selective electrodes are used for the determination of enzymes and substances which interact with enzymes.
Procedure
1. Preparation of Standards 2. Instrument Setup and Operation 3. Analysis of sample