MicroRNA analysis by ion mobility-mass spectrometry (CAT#: STEM-ST-0084-LJX)

Introduction

Liquid chromatography/mass spectrometry is widely used for studying sequence determination and modification analysis of small RNAs. However, the efficiency of liquid chromatography-based separation of intact small RNA species is insufficient, since the physiochemical properties among small RNAs are very similar. In this service, we focused on ion mobility-mass spectrometry (IM-MS), which is a gas-phase separation technique coupled with mass spectrometry; we have evaluated the utility of IM-MS for microRNA (miRNA) analysis.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Ion mobility spectrometry–mass spectrometry (IMS-MS) is an analytical chemistry method that separates gas phase ions based on their interaction with a collision gas and their masses. In the first step, the ions are separated according to their mobility through a buffer gas on a millisecond timescale using an ion mobility spectrometer. The separated ions are then introduced into a mass analyzer in a second step where their mass-to-charge ratios can be determined on a microsecond timescale.

Applications

For studying the gas phase ion structure
For detecting the chemical warfare agents and explosives
For the analysis of proteins, peptides, drug-like molecules and nano particles
For monitoring isomeric reaction intermediates and probe their kinetics
For proteomics and pharmaceutical analysis

Procedure

1. Add sample
2. The ions in the sample are separated in the ion mobility spectrometer
3. The separated ions are introduced into the mass analyzer for detection
4. Store the detection results

Materials

• Sample Type:
MicroRNA

Notes

1. Ion mobility spectrometry is also a very fast technique, making it suitable for high-throughput applications. The entire analysis can be completed in just a few minutes.
2. The method is extremely sensitive and able to detect trace amounts of contaminants that other spectrometry methods would miss.
3. The effective separation of analytes achieved with this method makes it widely applicable in the analysis of complex samples such as in proteomics and metabolomics.

Other recommended products

Distinguishing between Phosphorylated and Nonphosphorylated Peptides by Ion Mobility−Mass Spectrometry (CAT#: STEM-ST-0148-LJX)
Separation and Characterization of Metallosupramolecular Libraries by Ion Mobility Mass Spectrometry (CAT#: STEM-ST-0142-LJX)
Both targeted and non-targeted analysis of phase II steroid metabolites in urine by ion mobility-mass spectrometry (CAT#: STEM-ST-0163-LJX)
Four-Dimensional Untargeted Profiling of N-Acylethanolamine Lipids in the Mouse Brain by Ion Mobility–Mass Spectrometry (CAT#: STEM-ST-0176-LJX)
Analysis of isomeric disaccharide precursor, product and cluster ions by ion mobility mass spectrometry (CAT#: STEM-ST-0160-LJX)
Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility (CAT#: STEM-ST-0122-LJX)
Thyreostatic Drug Detection in Animal Tissues by High-Field Asymmetric Waveform Ion Mobility Spectrometry-Mass Spectrometry (CAT#: STEM-ST-0093-LJX)
Characterizing the antibody by ion mobility mass spectrometry (CAT#: STEM-ST-0083-LJX)