Modified Cells Production

Cell modification refers to genetical alteration and reprogramming of cell function through introducing external cargos such as DNAs, RNAs, plasmid DNAs, proteins, or nanomaterials into the cytosol or nucleus of living cells. The process of external cargos introduction is called as intracellular delivery. Traditional methods for intracellular delivery such as bulk electroporation or viral vectors face a few drawbacks, such as low cell viability, cytotoxicity, and uneven material delivery, which has sparked interest in the creation of more effective intracellular delivery systems. Microfluidic devices offer a promising solution for intracellular delivery of genetic materials because of their low usage of materials, high throughput, high customizability, and flexibility. Microfluidic devices have been developed to deliver a wide range of different payloads into different cell types with the highest level of precision and efficiency.

What We Can Do for You

STEMart provides a versatile platform for modified cell production with enhanced efficiency and specificity with microfluidics technology.

• Isolation of desired cells from biological samples using microfluidic cell separation techniques based on mechanical, chemical, electrical, and magnetic principles
• Exploration of the effects of multiple factors such as three-dimensional culture, co-stimulatory factor type and concentration, and oxygen content on cell expansion
• Delivery of cargo molecule into cells for reprogramming of cell functions

Main microfluidic devices for intracellular delivery include:

• Microfluidic electroporation devices use an electrical field to create temporary pores in the cell membrane, allowing for the uptake of molecules.
• Microfluidic liposome delivery devices use liposomes, which are small vesicles made of lipid bilayers, to encapsulate and release cargo molecules into cells in response to specific stimuli, such as changes in pH or temperature.
• Microfluidic optoporation devices use light to disrupt the cell membrane and allow for the delivery of molecules.
• Microfluidic nanoparticle delivery devices use nanoparticles, such as gold or silica nanoparticles which is functionalized with targeting ligands to selectively deliver cargo to specific cells or tissues.
• High-throughput screening of successfully modified cells
• Expansion of modified cells in controlled microenvironment

Application of Modified Cells

STEMart can help customer produce desired modified cells for:

• Treatment of genetic disorders
• Adoptive cell therapy (ACT) for cancer treatment
• Organ transplantation
• Microorganisms engineering
• Plant engineering

For more information about our modified cells production service, please contact us.