Nanoscale organization of nicotinic acetylcholine receptors revealed by stimulated emission depletion microscopy (CAT#: STEM-MIT-0362-LJX)

Introduction

Nicotinic acetylcholine receptors (nAChRs), commonly referred to as ionotropic acetylcholine receptors (NachRs), develop specific responses to nicotine. Acetylcholine receptor (AChR) supramolecular aggregates that have hitherto only been accessible to examination by electron microscopy were imaged with stimulated emission depletion (STED) fluorescence microscopy, providing resolution beyond limits of diffraction of classical wide-field or confocal microscopes.

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Principle Applications Procedure Materials Notes Related Products

Principle

Stimulated emission depletion (STED) microscopy uses two light sources. One source emits light that excites the fluorophores, and the other emits a ring laser of different wavelengths, which is used to suppress fluorescence.

Applications

Imaging of the intensity distribution of the fluorescent sample
Imaging of living samples
Measuring of the fluorescence lifetime and fluorescence correlation spectrum of the fluorescent samples
Used in the fields of biology, medicine and materials science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Nicotinic acetylcholine receptors

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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