Nickel Chelating Resin, 3ml Spin Column, 5 Columns, 786-394, G-BIOSCIENCES (CAT#: STEM-C-3188-LGZ)

Immobilized metal affinity chromatography (IMAC) resin utilizes nickel (Ni2+) to purify 6-fold histidine-tagged proteins.

This resin binds six histidine residues (6x His), a common tag used for protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The binding capacity of iminodiacetic acid and divalent nickel ions is 20~40μmol/ml resin. The protein binding capacity is 50 mg protein per ml resin. We have demonstrated the binding of 100mg of 50kDa 6XHis-tagged protein to one milliliter of resin. Nickel NTA resin is also available.

The resin bed volumes of the spin columns were 0.2, 1 and 3 ml, respectively, and the total column volumes were 1, 8 and 22 ml, respectively. The column is available as a spin format for gravity flow columns.

Immobilized copper, cobalt and zinc chelating resins are also available. Cobalt has the highest selectivity, followed by zinc, nickel and copper, but has the lowest binding capacity. Copper has the highest binding strength, followed by nickel and then zinc.

Specific binding/wash and elution buffers are available.

Our new HOOK™ 6X His Protein Purification Kit is now available and includes everything needed to purify 6X His-tagged proteins from yeast or bacteria, including lysis buffer, lysing enzyme, resin, column and binding, wash and elution buffers . Each kit is available with nickel or cobalt chelate resins. Available kits are:

HOOK™ 6X His Protein Purification (Bacteria): Isolated from bacteria; optimized to produce up to 10mg/250ml of soluble 6X His-tagged protein.
HOOK™ 6X His Protein Spin Purification (Bacteria): Isolation from bacteria; optimized to produce ~1mg/50ml of soluble 6X His-tagged protein culture
HOOK™ 6X His Protein Purification (Yeast): Isolated from yeast; optimized to yield up to 10 mg of soluble 6X His-tagged protein.
HOOK™ 6X His Protein Spin Purification (Yeast): Isolated from yeast; optimized to yield up to 1 mg of soluble 6X His-tagged protein.

Size: 5 columns
For the purification of 6x His proteins
High capacity: >50mg/ml
Ligand density: 20-40μmoles Ni2+ /ml resin
Bead Structure: 6% cross-linked agarose

G-Sep™ Co IDA Agarose Fast Flow, 25ml, 786-978, G-BIOSCIENCES (CAT#-STEM-C-3168-LGZ)
G-Sep™ Co IDA Agarose Fast Flow, 100ml, 786-979, G-BIOSCIENCES (CAT#-STEM-C-3169-LGZ)
G-Sep™ Co IDA Agarose Fast Flow, 500ml, 786-980, G-BIOSCIENCES (CAT#-STEM-C-3170-LGZ)
G-Sep™ Ni IDA Agarose Fast Flow, 5ml, 786-973, G-BIOSCIENCES (CAT#-STEM-C-3171-LGZ)
G-Sep™ Ni IDA Agarose Fast Flow, 25ml, 786-974, G-BIOSCIENCES (CAT#-STEM-C-3172-LGZ)
G-Sep™ Ni IDA Agarose Fast Flow, 100ml, 786-975, G-BIOSCIENCES (CAT#-STEM-C-3173-LGZ)