Nuclear System Yeast Library Construction to study protein-protein interactions (CAT#: STEM-MB-0015-WXH)

Introduction

The nuclear-protein yeast two-hybrid technique was first established by Fields et al. when studying the nature of yeast transcription factor GAL4, and has subsequently developed into a mature protein-protein interaction research tool with the characteristics of simplicity, sensitivity and reflecting the real situation of protein interactions in living cells.
Currently, there are two methods for yeast library construction, one is the SMART system, which adds a unique step to the existing yeast two-hybrid library construction process (BD MatchmakerTM library), resulting in a significant reduction in the high abundance of genes expressed in the library, thus making the library uniform in abundance and increasing the screening positivity rate; the second is the Gateway system, which uses the Gateway site-specific The second is the Gateway system, which uses the Gateway site-specific recombination technology (Cloneminer cDNA library construction kit) to construct libraries.