Peptide Library Screening to study proteins (CAT#: STEM-MB-0028-WXH)

• Screening of receptor ligands
• Drug peptide discovery
• Protein interactions
• Proteome function
• Peptide nucleic acids
• Screening for enzyme substrates or peptidase inhibitors
• Antigen binding site or antigen epitope mapping
• Discovery of T-cell epitopes
• Antibody binding sites on antigens (proteins)
• Cross-reactivity of antibodies with related proteins
• Identification of short peptide antigens

1. Truncation peptide libraries：It is used to identify the shortest amino acid sequence that plays an active role in the peptide sequence, and the truncation peptide libraries are generally built by systematically removing amino acids from both sides of the original peptide sequence.
2. Random Peptide Library: It is designed by replacing the selected amino acid residues with 20 natural amino acids simultaneously and randomly by the shotgun approach.
3. Ala Scanning Peptide Library: It is a way to identify the effect of a specific amino acid in a sequence on peptide efficacy, using Ala to replace amino acids in sequence in turn.
4. Scrambled Library: It is designed based on the internal replacement of the original peptide amino acid sequence. It is the most mutated peptide library and a random screening tool used to discover new targets.
5. Overlapping Library: It is mainly used for whole protein scanning and provides an ideal tool for screening linear or continuous epitopes. It is designed to gradually intercept from the N-terminal to the C-terminal of the target protein, transferring one or several amino acids at a time, with partial overlap between sequences, eventually forming an overlapping peptide library.