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Full-Length cDNA Library Construction to study expression status of genomes (CAT#: STEM-MB-0035-WXH)

Introduction

A cDNA library is a collection of clones formed by the reverse transcription of all mRNAs transcribed during a developmental period and ligated to a vector.
A full-length cDNA library is a population of DNA subunits obtained by reverse transcription from a complete set of mRNA subunits in an organism, which is a complete copy of the mRNA subunits. Full-length cDNA libraries not only provide complete mRNA information, but also can be used to obtain mRNA splicing information by gene sequence comparison, protein sequence prediction and in vitro expression, and gene function study by reverse genetics.




Applications

• Study of the expression status of genomes in specific cells
• Functional identification of expressed genes

Procedure

1.Total RNA extraction
2.Isolation of mRNA
3.Perform full-length mRNA enrichment
4.Synthesis of cDNA duplexes using mRNA as template
5. Perform graded purification of the synthesized cDNA duplexes.
6. Perform all-direct recombination reaction of the graded purified cDNA with vector.
7. Transform the recombinant product into receptor cells.
8.Test the library quality

Notes

Customer provides tissue or RNA samples
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