Phage Display Library Construction and Screening for protein interaction studies (CAT#: STEM-MB-0039-WXH)

Introduction

Phage display technology is one of the major approaches employed in various protein interaction studies, especially in the discovery of specific antibodies, scaffolds or ligands. While binders with the desired specificity generally exist at low frequencies in the constructed libraries. In this way, phage display library screening has become an effective technique for the enrichment and identification of binders with high affinity and specificity from interested libraries.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Analysis of protein binding properties
• Monoclonal antibody specificity profiling
• Analysis of protein post-translational modifications
• Establishment of antigen-antibody library
• Pathogen detection
• Gene therapy
• Research on host-microbe interactions
• Biomarker identification
 Cell signaling research

Procedure

1.Construction of phage display library
Aimed genes are inserted into the multiple phages by recombinant DNA technology so that the protein will be displayed outside of phage particles.
Optional Equipment: Electroporator, Centrifuge, Shaker, Incubator, Lab Automated Extraction Workcells
2.Binding target proteins
3.Washing and elution
4.Amplification
5.DNA sequencing
The DNA within the interacting phage is sequenced to identify the interacting proteins or protein fragments.

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