Shotgun Library Construction for DNA sequencing (CAT#: STEM-MB-0038-WXH)

Introduction

A shotgun library is a collection of random-sheared DNA molecules. These libraries typically consist of inserts ranging from 1-10kb and extensively used in DNA sequencing, gene screening and DNA mapping throughout the world.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Applications

• Small genome sequencing such as virus, phage, plasmid, etc.;
• BAC/Fosmid clone sequencing;
• Specific genes’ identification;
• Gene screening;
• Thorough sequencing with fragment lengths > 9 k and without reference sequence.

Procedure

1.High quality DNA preparation.
2.Random shearing to produce a population of DNA fragments.
3.Ligation into a standard cloning vector and transformation.
4.Verification of library quality by randomly picking clones and determining insert sizes by restriction digest or DNA sequencing.

Notes

Customer provides adequate amount of fresh samples (animal or plant tissue or genomic DNA)

Other recommended products

Yeast Two-hybrid (Y2H) Library Construction to study protein-protein interactions (CAT#: STEM-MB-0013-WXH)
Membrane System Yeast Library Construction to study interactions of membrane proteins (CAT#: STEM-MB-0014-WXH)
Bacterial Two-hybrid (B2H) System to study protein-protein interactions (CAT#: STEM-MB-0019-WXH)
Alanine Scanning Library Construction to characterize structure-function relationships (CAT#: STEM-MB-0025-WXH)
Reverse Yeast Two-hybrid (rY2H) Library Construction to study protein-protein interactions (CAT#: STEM-MB-0021-WXH)
Phage Display Library Construction and Screening for protein interaction studies (CAT#: STEM-MB-0039-WXH)
Suppressor-Subtractive Hybridization (SSH) Library Construction to study gene expression differences (CAT#: STEM-MB-0020-WXH)
Yeast Display cDNA Library Construction to identify proteins (CAT#: STEM-MB-0034-WXH)