Shotgun Library Construction for DNA sequencing (CAT#: STEM-MB-0038-WXH)

Introduction

A shotgun library is a collection of random-sheared DNA molecules. These libraries typically consist of inserts ranging from 1-10kb and extensively used in DNA sequencing, gene screening and DNA mapping throughout the world.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Small genome sequencing such as virus, phage, plasmid, etc.;
• BAC/Fosmid clone sequencing;
• Specific genes’ identification;
• Gene screening;
• Thorough sequencing with fragment lengths > 9 k and without reference sequence.

Procedure

1.High quality DNA preparation.
2.Random shearing to produce a population of DNA fragments.
3.Ligation into a standard cloning vector and transformation.
4.Verification of library quality by randomly picking clones and determining insert sizes by restriction digest or DNA sequencing.

Notes

Customer provides adequate amount of fresh samples (animal or plant tissue or genomic DNA)

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