Yeast Display cDNA Library Construction to identify proteins (CAT#: STEM-MB-0034-WXH)

Introduction

Complementary DNA (cDNA) is the reverse transcript of mRNA. As lacking the large non-coding introns compared to genomic DNA, eukaryotic cDNA libraries represent the coding sequence of all temporally transcribed genes in a certain cell type. Heterologous protein fragments can be efficiently displayed at high copy levels on the Saccharomyces cerevisiae cell wall via yeast display system. Yeast display is widely used to screen large expressed protein libraries for proteins or protein fragments with specific binding properties.
Recently, yeast surface displayed cDNA libraries have been used to identify proteins which bind to various target molecules such as peptides, antibodies, and small molecules. Since yeast protein expression pathways are like those found in mammalian cells, human protein fragments displayed on the yeast cell wall are most likely to be properly folded and functional. Combined with fluorescence-activated cell sorting (FACS), yeast display cDNA libraries allow the selection of protein fragments or domains with affinity for any soluble molecule that can be fluorescently detected.