Observation of synaptic ultrastructure in mouse hippocampal CA1 region by transmission electron microscopy technology (CAT#: STEM-MIT-0415-LJX)

Introduction

Synapses are the structural basis of information exchange between neurons and between neurons and between effector cells. Changes in neural activity can be reflected in synaptic structure, such as changes in synaptic plasticity during learning and memory. Observation of synaptic morphology under electron microscope is the gold standard to identify synaptic structure. The ultrastructure of synapses can be observed under transmission electron microscope, including presynaptic components, synaptic cleft and postsynaptic components. Presynaptic components include synaptic vesicle and presynaptic membrane, which are closely related to the release and reabsorption of neurotransmitters. The most concerned of postsynaptic components is the postsynaptic density(PSD). It contains postsynaptic membrane, various neurotransmitter receptors and cytoskeleton structures, which are directly related to postsynaptic effects.
Adult hippocampal neurons play a major role in learning and memory. By observing the structure of the hippocampus of adult mouse, we can conduct neurobiological research on learning and memory.

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Principle Applications Procedure Materials Notes Related Products

Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.2nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample. In the field of neuroscience, transmission electron microscopy is an important means to observe the morphology and structure of synapses. The observation results can be directly used to analyze the synaptic function, such as the density of synapses, the morphology of vesicles in presynaptic components, the number of vesicles, the distribution characteristics of vesicles, and the thickness of postsynaptic dense matter.

Applications

Imaging and observation of mouse nerve tissue section samples

Procedure

1. Sampling
2. Preparation of slices
3. Staining
4. Observation

Materials

• Sample Type:
Hippocampal tissue of adult mouse

Notes

Tissue fixation and sampling are the most important preliminary steps, and the brain tissue must be adequately irrigated to remove residual blood.

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