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Observation of yeast saccharomyces cerevisiae by transmission electron microscopy technology (CAT#: STEM-MIT-0016-LJX)

Introduction

Saccharomyces cerevisiae belongs to the yeasts family, is a single-celled organism, oval or spherical. It has three ways of reproduction, respectively budding reproduction, spore reproduction, conjugation reproduction. Its form is simple but physiological complex, industrial used in wine.




Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Saccharomyces cerevisiae

Notes

Pay attention to air humidity
Voltage needs to be stabilized
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