Analyzing of lysosome by transmission electron microscopy technology (CAT#: STEM-MIT-0013-LJX)

Introduction

Lysosomes are organelles that break down proteins, nucleic acids, polysaccharides and other biological macromolecules. It has a single layer membrane, a variety of shapes, is 0.025 ~ 0.8 micron bubble structure, containing many hydrolase.
The function of the lysosome in the cell is to decompose the substances entering the cell from the outside. It can also digest the local cytoplasm or organelle of the cell itself. When the cell is aging, the lysosome breaks and releases hydrolytic enzymes to digest the whole cell and cause its death.

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Principle Applications Procedure Materials Notes Related Products

Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Lysosome-related organelles

Notes

Pay attention to air humidity
Voltage needs to be stabilized

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