Platelet-derived GF (PDGF) detection service (CAT#: STEM-MB-0256-WXH)

PDGF must bind to the corresponding receptor on the cell membrane to exert its biological effects. PDGF receptor is composed of two subunits α and β, and its molecular weight is 170-180KD. The binding between the two and PDGF is very different. The α unit has higher affinity for the PDGFa chain and the B chain, while the β subunit has only high affinity for the B chain. Therefore, the α subunit can bind to PDGF-AA, PDGF-AB, and PDGF-BB, and the β subunit can only bind to PDGF-BB and PDGF-AB. After PDGF binds to the receptor, PDGFR containing tyrosine kinase activity is dimerized and its tyrosine residue is autophosphorylated. The activated PDGFR binds to Grb2 to activate Ras, and then Raf-1, MEK, and ERK are successively activation. Nuclear transfer of activated ERK can induce phosphorylation of multiple transcription factors and regulate cell proliferation.

The biological activity of PDGF mainly includes 4 aspects.
First, it has chemotactic activity. PDGF induces migration of macrophages and fibroblasts, and has chemotaxis to neutrophils, smooth muscle cells, and fibroblasts.
Second, it has vasoconstrictive activity. In the early stage of trauma, it can stimulate the capillary of the wound site to contract rapidly, reduce the blood pressure and flow rate of the wound site, promote blood coagulation, and create conditions for wound repair.
Third, it has the mitogenic effect. PDGF can stimulate the proliferation and proliferation of vascular smooth muscle cells, fibroblasts and glial cells.
Fourth, it is involved in phosphatase activation and prostaglandin metabolism.

1. Process samples.
2. PDGF detection (Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Sample Types-Blood, serum, plasma, cell culture supernatant, cell lysate, cell culture medium, tissue homogenate, urine, tumor, etc.