IL-27 Detection (CAT#: STEM-MB-0294-WXH)

Introduction

IL-27, a member of the IL-12/IL-6 family, is produced primarily by antigen-presenting cells and is highest in activated monocytes, monocyte-derived dendritic cells, and activated macrophages. It is expressed in bone marrow cells, dendritic cells (DCs) activated by lipopolysaccharide (LPS), monocytes and macrophages.IL-27 is a heterodimer composed of two polypeptide chains, EBI3 and p28. It can secrete γ-interferon, promote the differentiation of Th1 cells, inhibit the differentiation of Th17 and Th2 and induce regulatory T-cell activity, etc. It plays an important role in the development of inflammatory diseases, anti-tumor immunity and the maintenance of the stability of the internal environment of the body.

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Principle Applications Procedure Materials Notes Related Products

Principle

The receptor for IL-27 consists of WSX-1h and gb130. The WSX-1 molecule is mainly expressed in peripheral blood lymphocytes, thymus and spleen, with high expression on CD4+ T cells and natural killer (NK) cells, but also on mast cells, monocytes, DCs, and natural killer T cells. WSX-1 and gb130 contain many JAKs-related targets. After binding to the receptor, WSX-1 activates JAK1/2 and then participates in the differentiation of various immune cells through the downstream signal transducer and activator of transcription 1 and STAT3 pathways. IL-27 selectively activates STAT3 in fully activated CD4 T cells and activates both STAT1 and STAT3 in early activated T cells.

Applications

IL-27 plays an important role in the development of inflammatory diseases, anti-tumor immunity and the maintenance of the stability of the internal environment of the body.
IL-27 has the ability to promote the activation and proliferation of T cells, NK cells, mast cells and monocytes to produce inflammatory cytokines and promote the inflammatory response.

Procedure

1. Process samples.
2. IL-27 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Notes

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.

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