Akt Pathway Assay (CAT#: STEM-MB-0336-WXH)

Akt acts on the TSC1/TSC2 complex and mTOR signaling pathway to regulate cell growth; acts on CDK's inhibitory molecules P21 and P27, and indirectly affects the expression levels of cyclinD1 and p53 to regulate cell cycle and cell proliferation. Akt can promote cell survival by directly inhibiting pro-apoptotic signals such as pro-apoptotic regulators Bad and Forkhead family transcription factors. The transport of T lymphocytes to lymphoid tissues is controlled by the expression of adhesion factors downstream of Akt. Akt can also regulate neuronal function-related proteins such as GABA receptor ataxin-1 and huntingtin molecules. Akt has also been found to bind to Smad molecules to regulate TGFβ signaling. Akt also plays a role in the structural organization of nucleoprotein by phosphorylation of lamin A antigen.

To study the influence of each virus on Akt cell signaling pathway
To study the regulatory mechanism of Akt cell signaling pathway in diseases
To study the effects of drugs or therapies on Akt cell signaling pathway

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Detectable targets: JAK1, TSC1, TSC2, ATG13, Gab1, Gab2, Lyn, Tpi2, AS160, PIP5K, GS, MDM2, GSK3, GSK3, Wee1, Myt1, PFKFB2, Ataxin-1, eNOS, BcI-2, PDCD4, S6K, XIAP, Bim, Bax, PTEN, PP2A, PHLPP, CTMP, BCAP, IRS-1, IKK-α, p21, p70, SyK, p53, PI3K, p27