Akt Pathway Assay (CAT#: STEM-MB-0336-WXH)

Introduction

AKT, also known as protein kinase B (PKB) or RAS-alpha, is a ubiquitous serine/threonine kinase that plays an important role in a variety of biological reactions such as metabolism, regulation of cell survival and growth. This protein kinase is activated by insulin, PI3K, IGF1 and other growth and survival factors. AKT inhibits cell apoptosis by inhibiting phosphorylation and inactivating multiple targets including forkhead transcription factors and caspase-9, thereby promoting cell survival. The AKT pathway is the main goal of cancer drug discovery research. Invitrogen AKT signaling pathway antibodies are designed to reliably detect key AKT targets. Each antibody has been validated for various applications.

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Principle Applications Procedure Materials Notes Related Products

Principle

Akt acts on the TSC1/TSC2 complex and mTOR signaling pathway to regulate cell growth; acts on CDK's inhibitory molecules P21 and P27, and indirectly affects the expression levels of cyclinD1 and p53 to regulate cell cycle and cell proliferation. Akt can promote cell survival by directly inhibiting pro-apoptotic signals such as pro-apoptotic regulators Bad and Forkhead family transcription factors. The transport of T lymphocytes to lymphoid tissues is controlled by the expression of adhesion factors downstream of Akt. Akt can also regulate neuronal function-related proteins such as GABA receptor ataxin-1 and huntingtin molecules. Akt has also been found to bind to Smad molecules to regulate TGFβ signaling. Akt also plays a role in the structural organization of nucleoprotein by phosphorylation of lamin A antigen.

Applications

To study the influence of each virus on Akt cell signaling pathway
To study the regulatory mechanism of Akt cell signaling pathway in diseases
To study the effects of drugs or therapies on Akt cell signaling pathway

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: JAK1, TSC1, TSC2, ATG13, Gab1, Gab2, Lyn, Tpi2, AS160, PIP5K, GS, MDM2, GSK3, GSK3, Wee1, Myt1, PFKFB2, Ataxin-1, eNOS, BcI-2, PDCD4, S6K, XIAP, Bim, Bax, PTEN, PP2A, PHLPP, CTMP, BCAP, IRS-1, IKK-α, p21, p70, SyK, p53, PI3K, p27

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