EGF/EGFR Signaling Pathway Assay (CAT#: STEM-MB-0320-WXH)

Epidermal growth factor (EGF) is one of the high-affinity ligands of EGFR. The EGF/EGFR system induces growth, differentiation, migration, adhesion, and cell survival through a variety of interacting signaling pathways. The binding of EGF to the extracellular domain of EGFR induces dimerization, activation of intrinsic kinase activity and subsequent autophosphorylation of EGFR at multiple residues in the cytoplasmic region. Activated EGFR recruits various cytoplasmic proteins that transduce and regulate EGFR function. EGFR also activates phospholipase C, and one of the PKC-regulated signaling modules in the EGFR pathway is the NFKB module. Other signaling modules activated by EGFR include FAK, JNK, p38MAPK, and ERK5 modules. EGFR induces the JNK pathway through activation of G proteins such as RAC and CDC42. As growth, differentiation and cell survival factors; mutation and overexpression of EGFR and defective regulation of its signaling pathways have been shown to be associated with tumorigenesis.

Study the regulatory mechanism of EGF/EGFR signaling pathway in medicine
Research on the influence of various pathogens on EGF/EGFR signaling pathway
Study the effect of clinical drugs on EGF/EGFR signaling pathway

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Detectable targets: Bcl-xl, ISGF3, TRAF-2, FADD, APAF-1, BID, Histone-H3, IRF5, MEK3, p38MAPK, SH2, TRAF6, IPS-1, mTOR, MSK2, PI3K, TBK1, Vav, BAK, Bax, ISRE, MYD88, Rac1, TLR4, PKR, TLR3, IAPs, tBID, SODD, Bad, FADD, IRF7, Mda-5, NFκB, RIG-1, TRAF3, PIDD, IRS2, MSK1, p65, Tak1, TRIF, RIP1, IRS1, MEK6, p50, SLP76, TRAM, GAS, IRF9, MEKK1, p38, RIP1, TRAF5