IL-12 Detection (CAT#: STEM-MB-0281-WXH)

Introduction

IL-12 is a Th1-type cytokine composed of two subunits p35 and p40. It has a wide range of immunoregulatory effects. It is mainly composed of antigen-presenting cells such as monocytes, macrophages, B cells, and dendritic cells. IL-12 mainly mediates cellular immunity, activates natural killer (NK) cells, enhances phytohemagglutinin (PHA) -induced lymphoblast proliferation, and promotes the differentiation of CD4 + initial T cells (Th0) into helper T cells (Th1).It can also promote the synthesis of interferon γ (IFN-γ), tumor necrosis factor α (TNF-α) and IL-2, inhibit Th2 synthesis of IL-4, IL-5 and IL-10, and selectively inhibit the induction of IL-4 Immunoglobulin E (IgE) synthesis. In addition, IL-12 can induce the production of other cytokines and exert a strong biological function, which plays an important role in inflammation and anti-tumor immunity.

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Principle Applications Procedure Materials Notes Related Products

Principle

The IL-12 receptor is a type I transmembrane glycoprotein. It consists of two polypeptide chains, β1 and β2. The β1 chain binds to the p40 subunit, and the β2 chain can bind to the structure in the p35 subunit or heterodimer. Any a single subunit binds to its ligand with low affinity. Only when the two are co-expressed can IL-12 high-affinity binding sites be generated. IL-12Rβ2 allows IL-12 to interact with its receptor during signal transduction, activates the mutual phosphorylated kinases (Janus Kinase) JAK2 and TYK2, recruits STAT, and causes it to undergo phosphorylation under the action of kinases JAK2 and TYK2. Then phosphorylated STAT4 forms a homodimer in the cytoplasm and enters the nucleus, regulating the expression of the gene of interest.

Applications

IL-12 mainly mediates cellular immunity, activates natural killer (NK) cells, enhances phytohemagglutinin (PHA) -induced lymphoblast proliferation, and promotes the differentiation of CD4 + initial T cells (Th0) into helper T cells (Th1).
IL-12 can promote the synthesis of interferon γ (IFN-γ), tumor necrosis factor α (TNF-α) and IL-2, inhibit Th2 synthesis of IL-4, IL-5 and IL-10, and selectively inhibit the induction of IL-4 Immunoglobulin E (IgE) synthesis.
IL-12 can induce the production of other cytokines and exert a strong biological function, which plays an important role in inflammation and anti-tumor immunity.
IL-12 is considered as a NK cell stimulating factor and has a strong immunomodulatory effect.

Procedure

1. Process samples.
2. IL-12 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Notes

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.

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