PROTEINDEX™ rProtein L Agarose 4 Fast Flow, 25 mL, 11-0214-025, Marvelgent biosciences (CAT#: STEM-C-3717-LGZ)

PROTEINDEX™ rProtein L Sepharose 4 Fast Flow is an affinity chromatography medium designed for simple one-step purification of immunoglobulins, as well as Fab, scFv immunoglobulins, from laboratory and process scale biological fluids and cell culture media fragment.

Protein L was first isolated from the surface of the strain Peptostreptoccus magnus, and now recombinant protein L is obtained through gene recombination technology. Unlike protein A and protein G, which bind primarily through the Fc region of immunoglobulins, protein L binds the variable region of antibody kappa light chains and binds a broader range of Ig classes and subclasses. Therefore, Protein L is particularly suitable for capturing antibody fragments of various sizes, such as Fab fragments, single-chain variable fragments (scFv) and domain antibodies (dAbs). Also suitable for purification of mouse cell IgM.

PROTEINDEX™ rProtein L Sepharose 4 Fast Flow utilizes the stable thioether linkage between rProtein L and cross-linked Sepharose beads and is optimized for high binding capacity of immunoglobulins.

Size: 25 mL
Ligand: Recombinant protein L produced in E.coli
Binding capacity: >15 mg human IgG/mL medium
Matrix Cross-linked: 4% agarose supplied as a 50% slurry
Particle size: 45 μm - 165 μm
Maximum pressure: 0.3 MPa (3 bar)
Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
Recommended flow rate: Gravity flow and medium pressure, 30 - 300 cm/hour
Storage temperature: 2 - 8°C
Storage buffer: 20% ethanol

Chromatography Column, Fixed Volume, 5-7bar, 8mm ID, 700mm length, 5bar, GB-8-700, G-BIOSCIENCES (CAT#-STEM-C-3578-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 8mm ID, 800mm length, 5bar, GB-8-800, G-BIOSCIENCES (CAT#-STEM-C-3579-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 8mm ID, 900mm length, 5bar, GB-8-900, G-BIOSCIENCES (CAT#-STEM-C-3580-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 8mm ID, 1000mm length, 5bar, GB-8-1000, G-BIOSCIENCES (CAT#-STEM-C-3581-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 10mm ID, 100mm length, 7bar, GB-10-100, G-BIOSCIENCES (CAT#-STEM-C-3582-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 10mm ID, 200mm length, 7bar, GB-10-200, G-BIOSCIENCES (CAT#-STEM-C-3583-LGZ)