Pyroptosis Analysis (CAT#: STEM-CBT-0013-WXH)

Introduction

Pyroptosis, also known as inflammatory necrosis, is a new type of programmed cell death discovered and confirmed in recent years, which is dependent on inflammatory caspases and induced by cutting Gasdermin-D (GSDMD) protein , after GSDMD is cleaved, the N-terminal domain is released, which has the activity of binding membrane lipids and opening holes in the cell membrane, resulting in changes in cell osmotic pressure. Release, accompanied by the release of a large number of pro-inflammatory factors.
To prove whether the induced cell death is pyroptosis, the following experiments are generally required:
(1) Cutting of GSDMD (can be detected by Western blot, immunofluorescent staining, etc.);
(2) Pathway-related gene detection (RT-PCR, Western blot, etc. can be used for detection);
(3) Detection of inflammatory factor levels (Western blot, ELISA, etc. can be used for detection);
(4) Detection of cell morphology and cell viability (can be detected by electron microscopy, CCK-8, etc.);
(5) Chromatin integrity detection (TUNEL, etc.);
(6) Optional immunohistochemical detection of histone expression