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Quantification of MSRV by RT-qPCR (CAT#: STEM-MT-0042-LGZ)

Introduction

HERV-W is a multilocus family of human endogenous retroviruses (HERVs) that have been found to play important roles in human physiology and pathology. Two particular members of the HERV-W family are of particular interest: ERVWE1 (encodes syncytin-1, a glycoprotein essential in placenta formation) and MSRV (multiple sclerosis-associated retrovirus , thought to play an important role in human pathology due to its increased expression in brain tissue and blood cells of patients with multiple sclerosis (MS). The high similarity between ERVWE1 and MSRV mRNA necessitates the need for a method that can specifically quantify MSRV expression in clinical samples.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Multiple Sclerosis-Associated Retrovirus

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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