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Analysis of LGALS8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0430-LGZ)

Introduction

Official Full Name: galectin 8
Also known as: Gal-8; PCTA1; PCTA-1; Po66-CBP
This gene encodes a member of the lectin family. Galectins are -galactoside-binding animal lectins with a conserved carbohydrate recognition domain. Lectins are involved in many fundamental functions, including development, differentiation, cell-cell adhesion, cell-matrix interactions, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumor tissues and appears to be involved in integrin-like cell interactions. Alternative splicing transcript variants encoding different isoforms have been identified.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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