Quantitative investigation of platelet quality by UV-Vis Spectroscopy (CAT#: STEM-MB-0889-WXH)

Introduction

The quality of platelets transfused is vital to the effectiveness of the transfusion. Freshly prepared, discoid platelets are the most effective treatment for preventing spontaneous hemorrhage or for stopping an abnormal bleeding event. Current methodology for the routine testing of platelet quality involves random pH testing of platelet rich plasma and visual inspection of platelet rich plasma for a swirling pattern indicative of;he discoid shape of the cells. Models based on Mie theory have been developed which allow for the extraction of quantitative information on platelet size, number and quality from multiwavelength UV/vis spectra. These models have been used to quantify changes in platelet rich plasma during storage.