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Real-time monitoring of yeast cell division by Dielectric spectroscopy (CAT#: STEM-ST-0365-WXH)

Introduction

Yeast cells are round to long cells that reproduce vegetatively by budding or germinate to produce a mycelium. Yeast cells divide as rapidly as once every 90 min under optimal laboratory conditions, through a process of budding in which smaller daughter cells pinch, or bud, off the mother cell. The common name “budding yeast” derives from this notable feature of cell division and distinguishes S.




Principle

The principle of this technique is to apply an AC signal of small amplitude to an electrode immersed into an electrolyte. The initial disturbance (applied) and the response of the electrode is compared by measuring the phase shift of the current and voltage components and by the measurements of their amplitudes.

Applications

Used to study the response of a sample subjected to an applied electric field of fixed or changing frequency. DS describes the dielectric properties of a material as a function of frequency.

Materials

Dielectric spectrometer