Recombinant Human B7-2 Protein, Seafrom (CAT#: STEM-CT-0131-ZJF)

The Recombinant Human B7-2 Protein manufactured by Seafrom is suitable for T-cell co-stimulation.
Leukocyte Differentiation Antigen 86 (CD86), also known as B Lymphocyte Activation Antigen B7-2, is a type I membrane protein and a member of the immunoglobulin superfamily. It is primarily expressed in dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be upregulated by interferon-gamma (γ-interferon). CD86 serves as a ligand for two different proteins on the surface of T cells: CD28 (for self-regulation and intercellular binding) and CTLA-4 (for attenuated regulation and cell detachment). CD86, in coordination with CD80, initiates T cell activation. Recent research suggests that B7-2 promotes the generation of mature APC subsets and enhances APC function and survival. Furthermore, B7 proteins participate in innate immune responses by activating the NF-κB signaling pathway within macrophages. Therefore, CD86 is considered a promising candidate for immunotherapy.
Storage Information:
The lyophilized (freeze-dried) form can be stored at 4°C for 24 months, while the reconstituted liquid can be stored at -20°C for 6-12 months, with a recommendation to avoid repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: CD86, B7-2, B70, CD28LG2, LAB72, MGC34413
Protein Sequence: The DNA sequence encodes human B7-2 (GenBank: AAH40261.1), expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant human B7-2 protein contains 221 amino acids, with a predicted theoretical molecular weight of 27.2 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: Immobilized human B7-2 (His tag) at 5 μg/mL (100 µL/well) can bind to human CTLA-4 (FC tag) in a linear binding range of 20-200 ng/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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