Recombinant Mouse CD27 Ligand/CD70 Protein, hFc Tag, Yisheng (CAT#: STEM-CT-0224-ZJF)

The Recombinant Mouse CD27 Ligand/CD70 Protein, hFc Tag manufactured by Yisheng is suitable for studying mouse CD27 Ligand/CD70-mediated immune responses.

Source: Mouse CD27 Ligand/CD70 Protein is expressed from HEK293 with hFc tag at the N-terminal. It contains Ser45-Pro195.[Accession | O55237]
Molecular Weight: The protein has a predicted MW of 43.41 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on SDS- PAGE result.
Endotoxin: <1EU per μg by the LAL method.
Purity: > 95% as determined by SDS- PAGE and HPLC
Activity: ELISA Data: Immobilized Mouse CD27, mFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 35.5ng/ml determined by ELISA.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 6.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Shipped with ice pack.
Stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 ℃ under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
For safety and health, please wear lab coats and disposable gloves for operation.

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