Recombinant Human CD47 Protein, Seafrom (CAT#: STEM-CT-0138-ZJF)

The Recombinant Human CD47 Protein manufactured by Seafrom is suitable for immune cell interactions and responses.
CD47 antigen, also known as antigenic surface determinant protein OA3, integrin-associated protein (IAP), and protein MER6, contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is widely distributed in normal adult tissues. It serves as the adhesive receptor for THBS1 on platelets and plays a role in cell adhesion and regulation of integrins. It is also involved in synaptic plasticity and memory formation in the hippocampus. CD47 acts as a receptor for SIRPA, and the binding of CD47 to SIRPA prevents the maturation of immature dendritic cells and inhibits the production of cytokines by mature dendritic cells. The interaction of CD47 with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell proliferation, and co-stimulates T-cell activation.
Storage information for lyophilized products: They can be stored at 4°C for 24 months, and the liquid after reconstitution can be stored at -20°C for 6-12 months, avoiding repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: Protein MER6, Integrin-associated protein, Antigenic surface determinant protein OA3, Leukocyte surface antigen CD47, CD47, MER6, CD_antigen: CD47
Protein Sequence: The DNA sequence encodes human CD47 (NCBI Reference Sequence: NP_942088.1), expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant human CD47 protein contains 121 amino acids, with a predicted theoretical molecular weight of 15.4 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity (ELISA): Immobilized anti-CD47 antibody at a concentration of 1 μg/mL (100 μL/well) can bind to human CD47 protein (His tag) in a linear range of 0.5-2 ng/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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