The Recombinant Human GITR Ligand Protein manufactured by Seafrom is suitable for immune cell activation and regulation. Tumor Necrosis Factor Ligand Superfamily Member 18 (TNFSF18), also known as Glucocorticoid-Induced TNF-Related Ligand (GITRL or GITR Ligand) and Activation-Inducible TNF-Related Ligand (AITRL), belongs to the tumor necrosis factor family. It is highly expressed in various tissues such as the small intestine, ovary, testis, kidney, and endothelial cells. TNFSF18/GITRL levels are up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18/GITRL functions as a costimulator, reducing the threshold for T-cell activation and promoting T-cell proliferation. It plays a crucial role in the interactions between activated T-lymphocytes and endothelial cells. Additionally, TNFSF18/GITR Ligand is involved in mediating the activation of NF-kappa-B, a transcription factor that regulates various cellular processes. The freeze-dried (lyophilized) form of the protein can be stored at 4℃ for up to 24 months. Once reconstituted, the liquid can be stored at -20℃ for 6-12 months, with precautions taken to avoid repeated freeze-thaw cycles.
Specification
Expression Host: Human HEK293 cells Source: Human Synonyms: TNFSF18, AITRL, TL6, hGITRL Protein Sequence: The DNA sequence encodes human GITR Ligand (GenBank: AAD22634.1), expressed with an Fc tag at the C-terminus. Molecular Weight: Recombinant human GITR Ligand protein contains 359 amino acids, with a predicted theoretical molecular weight of 41.2 kD. Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis. Endotoxin: <0.1 EU/μg Biological Activity: Immobilized human GITR (His tag) at 1 μg/mL (100 μL/well) can bind to GITR Ligand (Fc tag) in a linear range of 5-20 ng/mL. Purification Method: Chromatographic purification Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.
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