Recombinant SARS-CoV-2 Nucleocapsid Protein, Seafrom (CAT#: STEM-CT-0108-ZJF)

The Recombinant SARS-CoV-2 Nucleocapsid Protein manufactured by Seafrom is suitable for studying SARS-CoV-2 virus-host interactions.
The Coronavirus N protein is essential for coronavirus RNA synthesis and exhibits RNA chaperone activity, which may be involved in template switching. It is the most abundant protein in coronaviruses. The N protein's primary role is to package the positive-strand viral genome RNA into a helical ribonucleocapsid (RNP). Additionally, it plays a fundamental role during virion assembly by interacting with the viral genome and the membrane protein M.
The N protein also plays a critical role in enhancing the efficiency of subgenomic viral RNA transcription and viral replication. Due to the conserved sequence and strong immunogenicity of the N protein, it is commonly used as a diagnostic tool for coronaviruses.
Storage Information:
1. Lyophilized samples can be stored at 4°C for 24 months, and reconstituted liquid can be stored at -20°C for 6-12 months, while avoiding repeated freeze-thaw cycles.
2. Liquid samples can be stored at -20 to -80°C for 12 months, and after thawing, should be aliquoted and stored at -20°C for an additional 6-12 months, while avoiding repeated freeze-thaw cycles.

Synonyms: 2019-nCoV coronavirus NP Protein; 2019-nCoV coronavirus Nucleocapsid Protein; 2019-nCoVcovnp Protein; 2019-nCoV ncov NP Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein; 2019-nCoVnp Protein; 2019-nCoV nucleocapsid Protein
Expression Host: Human HEK293 cells
Protein Sequence: The DNA sequence encodes recombinant SARS-CoV-2 (2019-nCoV) N protein, expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant SARS-CoV-2 (2019-nCoV) N protein contains 432 amino acids, with a predicted theoretical molecular weight of 47.3 kD.
Purity: >90%, confirmed by SDS-PAGE gel analysis.
Endotoxin: ≤0.01 EU/μg (gel-clot method)
Purification Method: Chromatographic purification
Composition:
Freeze-dried powder of sterile PBS (pH 7.4) containing 6% mannitol and 6% trehalose.
Liquid of sterile PBS (pH 7.4) filtered through a 0.2 μM filter at a concentration of 0.2 μM.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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