Single-Cell Genome Sequencing

Single cell genome sequencing is a technique that allows researchers to analyze the genetic material of individual cells, rather than a population of cells. This high-resolution approach provides insights into the genetic diversity and heterogeneity within a complex sample, enabling researchers to identify rare cell types, detect genomic alterations, and study the dynamics of cellular processes at the single-cell level.

The process of single cell genome sequencing involves isolating and lysing individual cells, followed by the amplification of their DNA using techniques such as multiple displacement amplification (MDA) or polymerase chain reaction (PCR). The amplified DNA is then sequenced using next-generation sequencing technologies, generating a detailed profile of the genetic material within each cell. Traditional methods for single cell genome sequencing involves a series of complex and time-consuming steps, and are considered with several disadvantage such as limited throughput, sample contamination and high cost.

To overcome these problems, microfluidics technique is developed and increased applied in single-cell genome sequencing due to its ability to process small sample volumes, high sensitivity, and increased throughput.  Microfluidic devices use tiny channels and chambers to manipulate and analyze individual cells, enabling precise control over experimental conditions and reducing the risk of sample contamination.

What We Can Do for You

STEMart utilizes microfluidics technique to conduct single-cell genome sequencing to help out customers to complete their important research tasks which include but not limited to:

• Cancer research: Understand the genetic mutations and heterogeneity of cancer cells within a tumor, leading to more precise diagnosis, prognosis, and treatment strategies.
• Developmental biology: Gain insights into the processes of cellular differentiation and development, as well as the genetic drivers of disease.
• Microbial ecology: Identify and characterize individual microbial species in complex environmental samples, shedding light on microbial diversity, function, and interactions.
• Neurobiology: Uncover the genetic basis of neuronal diversity, plasticity, and function, and gain insights into neurological disorders.
• Reproductive biology: Study the genetic profiles of individual gametes and early embryos, improving our understanding of fertility, reproduction, and embryo development.
• Immunology: Study immune cell diversity, response, and dysfunction, leading to new insights into autoimmune diseases, cancer immunotherapy, and infectious diseases.

For more information about our single-cell genome sequencing service, please contact us.