Study membrane interactions of C-terminal peptides by Dual polarization interferometry (DPI) (CAT#: STEM-MB-0411-WXH)

Introduction

C-terminal peptides of human thrombin have been identified as a novel class of host defense peptides with antimicrobial as well as anti-inflammatory properties. Such peptides not only display potent antimicrobial action through direct membrane disruption, but also binding to lipopolysaccharide (LPS) from Gram-negative bacteria, and are able to reduce LPSinduced inflammatory responses, evidenced from NO production in macrophages, as well as from results in animal models of septic shock induced by LPS or bacteria.

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Principle Applications Procedure Materials Notes Related Products

Principle

Dual polarization interferometry (DPI) is an analytical technique that allows the simultaneous determination of thickness, density, and mass of a biological layer on a sensing waveguide surface in real time. DPI focuses laser light into two waveguides. One of these functions as the "sensing" waveguide having an exposed surface while the second one functions to maintain a reference beam. A two-dimensional interference pattern is formed in the far field by combining the light passing through the two waveguides. The DPI technique rotates the polarization of the laser, to alternately excite two polarization modes of the waveguides. Measurement of the interferogram for both polarizations allows both the refractive index and the thickness of the adsorbed layer to be calculated. These measurements can be used to infer conformational information about the molecular interactions taking place, as the molecule size (from the layer thickness) and the fold density (from the RI) change.

Applications

Investigation of the mechanisms underlying antimicrobial and anti-endotoxic effects for the C-terminal peptides.
Drug Discovery.

Procedure

1. Setting of dual polarization interferometry
2. Preparing the DPI sensor chip
3. Immobilization of target on DPI biosensor
4. Reagent was injected to react
5. Quantitative analysis

Materials

• DPI biosensor
• DPI sensor chip

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