Inquiry Basket

Study of electrophysiology in nodose ganglia of adult rat by Patch clamp (CAT#: STEM-PET-0057-WXH)

Introduction

Nodose ganglia are paired structures that receive cardiac afferent inputs from sensory neurites located in atrial and ventricular tissues. These sensory neurites preferentially sense chemical stimuli, with a few responding to mechanical stimuli or both modalities.

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

The patch-clamp technique involves a glass micropipette forming a tight gigaohm seal with the cell membrane. The micropipette contains a wire bathed in an electrolytic solution to conduct ions. To measure single ion channels, a “patch” of membrane is pulled away from the cell after forming a gigaohm seal.

Applications

• Study of ionic currents in individual isolated living cells, tissue sections, or patches of cell membrane.
• Study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells.
• Study of ion channels.

Procedure

1. Fabrication of glass electrodes
2. Measuring glass electrode resistance and compensating offset potential
3. Glass electrode contact to cell membrane and obtain a GΩ seal
4. Acquire and analyse recordings using the appropriate software.

Materials

Patch clamp system

Other recommended products

Study of ion transport in isolated plant vacuoles by Patch clamp (CAT#: STEM-PET-0032-WXH)
Study of Membrane properties of cholinergic neurons by Patch clamp (CAT#: STEM-PET-0070-WXH)
Analysis of granule cells of rat hippocampus by Patch clamp (CAT#: STEM-PET-0073-WXH)
Study of Cyclic Nucleotide-Gated Channels by Patch clamp (CAT#: STEM-PET-0052-WXH)
Study of human mitochondrial KATP channel by Patch clamp (CAT#: STEM-PET-0059-WXH)
Study of heptanol-induced uncoupling of cardiac gap junctions by Patch clamp (CAT#: STEM-PET-0044-WXH)
Drug discovery by Automated patch clamp by Patch clamp (CAT#: STEM-PET-0030-WXH)
Study of isolated mouse olfactory receptor neurons by Patch clamp (CAT#: STEM-PET-0050-WXH)

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies